منابع مشابه
Studies on Horseradish Peroxidase
The kinetics of the oxidation of p-cresol by Compound II of horseradish peroxidase has been studied by the stopped flow technique at an ionic strength of 0.11 from pH 2 to 11. In acid solution the reaction is kinetically first order in jcresol, but in the alkaline region a saturation effect attributable to complex formation is observed. At very high pH an additional second order reaction betwee...
متن کاملStudies on Cytochrome c Peroxidase
Apoproteins of cytochrome c peroxidase, horseradish peroxidase, and sperm whale myoglobin were recombined with manganese complexes of proto-, hemato-, meso-, and deuteroporphyrins to form manganese porphyrin-protein complexes. These complexes were purified by column chromatography. All the manganese porphyrin-containing cytochrome c peroxidases were crystallized. Light absorption maxima of mang...
متن کامل-Pectate Binding Site on an Apoplastic Peroxidase
An apoplastic isoperoxidase from zucchini (APRX) was shown to bind strongly to polygalacturonic acid in their Ca 2 1 induced conformation. By homology modeling, we were able to identify a motif of four clustered arginines (positions 117, 262, 268, and 271) that could be responsible for this binding. To verify the role of these arginine residues in the binding process, we prepared three mutants ...
متن کاملCovalent Immobilization of Sapindus Peroxidase on Polyaniline
Covalent immobilization of peroxidase is convenient in continuous processes to improve them economically. In the present study, peroxidases from Sapindus mukorossi were immobilized and used for removal of 4-chlorophenol (4-CP) by the polymerization pathway. Five milligram of vacuum dried polyaniline-glutaraldehyde composite was incubated in 1ml sodium acetate buffer (50mM;pH 5.0) containing 0.5...
متن کاملInfluence of dopamine on peroxidase-based assays.
During the investigation of discrepant laboratory results, we detected an unusual interference in several of the biochemistry tests. The patient’s specimen had been obtained from a catheter in the jugular vein. Results of all analytes were discordant from those for a sample drawn 10 h previously. The cause of the discrepancy was thought to be contamination with isotonic saline containing 100 g/...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1934
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)75353-2